The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis

英语. The filtrate antigen of Dermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immunodiffusion test and electrophoresis. Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals. The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep

印度尼西亚. Pembuatan antigen fitrat Dermatophilus congolensis mengikuti cara Makinde, sedangkan pembuatan antigen whole cell mengikuti cara Bida dan Kelley. Antigen fitrat Dermatophilus congolensis telah diuji dengan serum positif dari hewan percobaan marmot dan domba dengan uji imunodifusi dan elektroforesis. Serum positif dibuat dengan menyuntikkan antigen whole cell dari D. congolensis. Hasilnya menunjukkan bahwa imunodifusi agar membentuk masing-masing 1 dan 2 garis presipitasi dengan serum positif domba dan marmot. Pemisahan protein dari antigen dengan elektroforesis SDS-PAGE menunjukkan adanya 8 pita (band) dengan bobot molekul dari 30 kD sampai lebih dari 94 kD. Band kemudian ditransfer ke dalam membran nitroselulose dan bereaksi positif pada titrasi dengan serum positif domba