[Effect of branched chain amino acids supplement on fermentability and digestibility of palm press fibre]
2000
Zain, M. (Universitas Andalas, Padang (Indonesia). Fakultas Peternakan) | Sastradipradja, D. | Nur, M.A. | Suryahadi | Ramli, N.
Branched chain amino acids (BCAA) has been claimed to stimulate microbia growth in the rumen, hence and fiber digestion. Most of the works however, used Valine (Val), Leucine (Leu), or Isoleucine (Ile) as single supplement, it is known that the acids are antagonistic in the transfer into cells. What is the proper proportion of the acids has never been elucidated. This study tried to make use the ample supply of palm press fiber (PPF) as the source of roughage and to elucidate the best ratio of the BCAA for fermentability and digestibility improvement. The study was a randomized complete block in vitro experiment of 3 replicates with 3 at the power of 3 factorial treatments. The factors were the BCAA, supplemented into the basal diet, each at 0, 0.1 or 0.2 percent. Dry matter (DM) of the basal diet composed of 50 percent PPF, 25 percent wheat bran, 23.5 percent coconut oil meal, 0.5 percent limestone, 0.5 percent vitaminerals, 0.25 percent urea, and 0.25 percent common salt. The diet contained 5.18 MJ (Mega Joule) digestible energy (DE) and 16 percent crude protein. The BCAA supplementation improved (P less than 0.001) digestibility of DM (52.4 vs 60.9 percent) and organic matter (OM) (57.5 vs 64.7 percent). Supplementation of 0.1 percent Val + 0.2 percent Leu + 0.1 percent Ile resulted in the best DM (65.2 approx. 3.99 percent) and OM digestibility (69.3 approx. 4.14 percent), as well as volatile fatty acids (VFA) production (115 approx. 15 mM). The BCAA addition however, decreased (P less than 0,001) ammonia (3.47 vs 2.36 mM). Apparently it was used for de novo synthesis of microbial amino acids. Based on the response curve of digestibility variables to Ile levels of the 0.1 percent Val supplemented diets it is concluded that the best levels of BCAA for digestibility and fermentation improvement is 0.1 percent Val + 0.2 percent Leu + 0.12 percent Ile. Even it is not the best, the use of Ile as a single supplement at 0.2 percent is worth consideration
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