Marker-assisted varietal improvement of maize for yield and resistance to stalk rot complex
2002
Anon.
The project aimed to map bacterial rot resistant genes in maize, establish heterotic patterns of maize lines using molecular markers, isolate and characterize maize stalk rot pathogens occuring in corn-growing regions in the country using conventional and deoxyribonucleic acid (DNA) technologies and identify resistant corn inbreds to specific fungal and bacterial stalk rot pathogens. The project constituted the map for stalk rot resistant population. It produced second generation hybrid (F2) and backcross, first generation hybrid (BCF1) mapping populations of three out of four selected R x S crosses, and repeated the cross P8 x Y1F. Simple sequence repeats (SSR) fingerprinting of 81 yellow and maize inbred lines was also done. Agronomic performance trial of 39 F18 and parents is ongoing. The project was able to generate diallel crosses, produce antiserum for Erwinia chrysanthemi, and characterize for virulence 50% of 102 isolates collected. The initiated preliminary analysis used E. chrysanthemi specific virulence gene primers ADE1/ADE2
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