Effect of environmental factors on mycelial growth, spore germination and Pycnidial production of Stenocarpella macrospora (Earle) Sutton causing leaf blight, stalk rot and ear rot in corn
2002
Alovera, R.B. (Central Mindanao Univ., Kabacan, Cotabato (Philippines) Ilag, L.L. | Raymundo, A.D.
Laboratory experiments were conducted to determine the effect of environmental factors on mycelial growth, spore germination and pycnidial formation of Stenocarpella macrospora (Earle) Sutton. Mycelial growth and pycnidial formation of the fungus were affected by culture medium, light, temperature and pH. Luxuriant mycelial growth was observed in all media exposed under different light conditions with oatmeal agar as the best. Submerged pycnidia were formed singly in carrot agar, corn meal agar and V-8 juice agar but were formed as surface aggregates on oatmeal agar. On malt extract agar and potato dextrose agar, the fungus did not produce pycnidia under various light conditions. The optimum temperature for mycelial growth, pycnidial formation, conidial germination and discharge of conidia from pycnidia was from 20 to 30 deg C. At higher temperature, the conidia need more moisture to trigger germination. The fungus has a wide pH range favorable for growth from pH 3.5 to pH 5.85. Numerous pycnidia were formed in media with added lactic acid (pH 5.65) higher than those adjusted with the addition of NaOH and HCl
显示更多 [+] 显示较少 [-]