In vitro shoot tip culture of sugar-cane (Saccharum officinarum) variety Isd 28
2002
Baksha, R. | Alam, R. | Karim, M.Z. | Paul, S.K. | Hossain, M.A. | Miah, M.A.S. | Rahman, A.B.M.M. (Bangladesh Sugarcane Research Inst., Pabna (Bangladesh). Biotechnology Lab.)
Multiple shoots were obtained from shoot tip explant of sugar-cane (Saccharum officinarum) cultured on MS medium supplemented with BAP (0.5-2.0 mg L-1), Kn (0.1-0.5 mg L-1) and IBA (0.1-0.5 mg L-1). Roots were induced in vitro regenerated shoots on half MS medium supplemented with 5.0 mg L-1 NAA, IBA and IAA. Plant regeneration from shoot tip was the highest on MS medium supplemented with BAP 2.0 mg L-1 and IBA 0.5 mg L-1. The reported experimental findings present a method of plant regeneration of sugar-cane variety Isd 28 through shoot tip culture. The plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 70.
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