Bioconversion of filter press cake (mud) of sugar-cane to biomass protein and its biological evaluation
2002
Baig, T.T. | Sheikh, M.A. | Hameed, A. | Jamil, A. | Batool, F. | Ali, S.M. | Ali, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry)
Filter Press Cake (Mud) was enriched with biomass protein using Arachniotus sp. as fermentative organism. The Mud was dewaxed for increased protein production before fermentation. Proximate analysis of native and biomass was done. It was found that after fermentation ash contents decreased from 17.50 to 16.00 % and fiber was decreased from 28 to 2%. Ether extract remained the same. Prior to the production of biomass protein certain conditions like fermentation period (72 h), substrate to water ratio (6%), (NH4)2SO4(0.1 %), CaC12.2H2O (0.025%), MgSO4.7H2O (0.015%), KH2PO4 (0.1 %), Cane molasses (1.5%), and Corn Steep Liquor (2.0%) were optimized. These conditions were then applied to produce biomass protein on large scale. The biomass thus produced contained 26.25% crude protein, 13.12% true protein, 6.73% ether extract, 16% ash and 2% crude fiber. Amino acid analysis of biomass revealed the presence of 15 amino acids. The chemical score of protein was '0' and Leucine and Valine were first and second limiting amino acids respectively. The protein quality of biomass was tested in terms of digestibility that showed an average digestibility of 80.917%.
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