In vitro shoot development from juvenile cuttings of field-grown olive (Olea europaea L.) cv. Leccino
2002
Khan, M.R. | Rashid, H. | Quraishi, A. (National Agricultural Research Centre, Islamabad (Pakistan). Agricultural Biotechnology Inst.)
Effects of different growth regulators on uni-nodal explants of field-grown olive (Olea europaea L.) cv. Leccino were investigated. Single node cuttings were obtained from 8th bud pair. Disinfection protocols were standardized by using 0.1% mercuric chloride for five min followed by 50% Clorox for 15 min. sterilized cuttings were tested on MS medium supplemented with various plant growth regulators viz. gibberellic acid kinetin benzylaminopurine, and isopentenyladenine. These growth regulators were used singly in different combinations ranging from 1.0 to 8.0 muM l-1 In vitro. GA3 at a rate of 4 muM l-1 was found to be more effective in enhancing shoot elongation. A higher concentration of kinetin, RAP and 2iP induced more callusing and hyperhydrated microshoots. Inclusion of polyvinylpyrrolidone at a rate of 0.2 g/l in media was found to be successful to eliminate phenolic exudates.
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