In vitro rapid clonal propagation of an ornamental plant-Ixora fulgens Roxb.
2002
Amin, M.N. | Ahmad, S. | Sultana, S. | Alam, M.R. | Azad, M.A.K. (Rajshahi Univ. (Bangladesh). Dept. of Botany)
The cultures of proliferating axillary shoots were established on MS medium with different concentrations of either BA alone or in combinations of BA with NAA or IBA and with shoot explants from field grown mature plant of Ixora fulgens. Nodal segments were found to be the best explants for axillary shoot formation on agar gelled MS medium with 0.5 mg l-1 BA + 0.1 mg l-1 NAA. On this medium axillary buds showed sprouting within two weeks of incubation. By sequential re-culturing and sub-culturing 15-20 usable shoots (grater than 1 cm length) could be produced from the 2-node shoot segments after 12-15 weeks of culture. Micro-cuttings taken from In vitro proliferated shoots were rooted on 1/2 strength MS medium having 0.1-0.5 mg l-1 of either NAA, IBA and/or IAA. It was found that 90% of the plantlets could be established under Ex vitro conditions when they were transferred on specially made plastic tray containing coco-peet as potting mix.
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