The use of microsatellite analysis for verification of cultivar identity and homogeneity in bulk samples from grape plant material
2003
Hvarleva, Tz. | Tsvetkov, I. | Atanassov, A. | Atanassov, I. (AgroBioInstitute, Sofia (Bulgaria))
The development and application of reliable and low cost testing procedure for verification of cultivar identity and homogeneity is crucial for the large-scale production of certified grape plant material. In order to evaluate the sensitivity of microsatellite analysis of bulk probes from grape plants, a model system consisting of mixtures of leaf samples from two cultivars in different weight ratios was study. The obtained results demonstrated that microsatellite analysis could be efficiently applied for analysis of cultivar identity and homogeneity of bulk leaf probes from 20-25 grape plants. The possibilities for practical application of the microsatellite analysis for screening of nurseries and butches of cuttings is discussed.
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