Influence of Simazine and Atrazine as a source of carbon and nitrogen on number and growth of microflora in soil
2003
Milicic, B. | Radin, D. | Josic, D. (Institute of Soil Science, Belgrade (Serbia and Montenegro))
One of the basic criteria for the determination of herbicide effect on a biological activity of soil is their influence on number of soil microflora. Ever increasing application of Simazine and Atrazine in contemporary agricultural production created the need for investigation of their influenece on microflora in soil and ability of microorganisms in soil to use them as a sole or additional source of carbon and nitrogen was also under examination. Our investigations indicated higher number and more successful growth of microorganisms originated from previously Simazine and Atrazine-treated soil comparing to untreated soil, grown on nutrient media. They used these herbicides as a sole or additional source of carbon, nitrogen or both of them. The number of microorganisms from Simazine-treated soil was greater than that of microorganisms from Atrazine-treated soil. There was a greater reduction in number of microorganisms as well as in their growth of the media containing Simazine, particularly Atrazine as a sole source of carbon in relation to nutrient media in which these herbicides served as a sole source of nitrogen. The least number of microorganisms as well as the lowest growth rate were recorded in treated, particularly untreated soils on a medium containing Simazine and Atrazine as a sole source of carbon and nitrogen. Yeast extract, as an additional source of biotic substances reduced amount of sucrose and NH4NO3 as additional sources of carbon and nitrogen, had a stimulus effect on incidence and growth of microorganisms both in treated and untreated soils.
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