In vitro micropropagation of a medicinal plant species Sophora flavescens
2003
Zhao, D.L. | Guo, G.Q. | Wang, X.Y. | Zheng, G.C. (Lanzhou Univ. (China). The Inst. of Cell Biology)
A micro-propagating system based on the young stem node segments of Sophora flavescens was established. Murashige and Skoog (MS) basal medium supplemented with 8.88 microM 6-benzyladenine (BA) plus 2.69 microM alpha-naphthalene acetic acid (NAA) and that with only 5.37 microM NAA were found the best in promoting proliferation of shoots and induction of root, respectively. Percentages of shoot induction and number of shoot per explant were up to 93.4 % and 4.2 and rooting rate to 82.4 %, respectively. The segments of the regenerated shoots could be continuously induced to reproduce new shoots through subculture on the same medium in 30-d intervals and still kept this activity after being subcultured for 6 generations. After the regenerated plantlets were transplanted to field plots, they grew well, showing no any visible abnormalities.
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