Nucleotide-dependent isomerization of glutamate dehydrogenase in relation to total RNA contents of peanut
2003
Osuji, G.O. | Madu, W.C. | Braithwaite, C. | Beyene, A. | Roberts, P.S. | Bulgin, A. (Prairie View A and M Univ. (USA). Cooperative Agricultural Research Center)
The physiological function of glutamate dehydrogenase (GDH) was investigated by treating germinating peanut (Arachis hypogaea) seeds with nucleoside triphosphate (NTP) solutions in order to alter the isoenzyme distribution patterns. The free nucleosides and nucleotides of the GTP-treated peanut were the highest [8.7 micromol/g (f.m.)], and they decreased through the ATP-treated peanut [5.8 micromol/g (f.m.)], and CTP-treated peanut [5.5 micromol/g (f.m.)], to the UTP-treated peanut [4.1 micromol/g (f.m.)]. The combination of 4 NTPs induced 20 % higher content of Pi than in the control, but the combined ATP+UTP treatment induced the lowest Pi. The 4 NTP treatment also induced the highest number of GDH isoenzymes (28) followed by the purine NTP treatments (15 to 20). The deamination/amination ratios were generally higher in the UTP and CTP treated peanuts than in the GTP and ATP treated peanuts. There were mutual relationships between higher numbers of GDH isoenzymes present in the GTP-, and ATP-treated peanuts and higher RNA contents on one hand, and between the lower numbers of isoenzymes in the CTP-, and UTP-treated peanuts and lower RNA contents. The recurrent relationships of the effects of the NTP treatments of peanut were UTPATPCTPGTP.
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