Effect of 6-benzyladenine and casein hydrolysate on micropropagation of Amorpha fruticosa
2003
Gao, H.H. | Li, W. | Yang, J. | Wang, Y. | Guo, G.Q. | Zheng, G.C. (Lanzhou Univ. (China). Inst. of Cell Biology)
Using apical and axillary nodes as explants, a rapid and efficient method for propagation of Amorpha fruticosa has been developed. When grown on Murashige and Skoog (MS) medium supplemented with 8 mg/cubic dm benzyladenine, all explants responded with 4.94 shoots per explant after 6-weeks culture. Explants taken from the in vitro proliferated shoots subsequently produced multiple shoots when cultured on the same medium. The addition of casein hydrolysate (200 mg/cubic dm) enhanced the number of shoots up to 8.77 per subculture, and coconut milk was found to promote the shoot elongation and make them grow more vigorously, 82.53 % of excised shoots were rooted on half-strength MS medium containing 2.0 mg/cubic dm indoleacetic acid after 3 weeks of incubation. After acclimatization, all of the rooted plantlets established in soil, exhibiting uniform morphological and growth characteristics.
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