Hydroxymethylfurfural in Czech honeys
2003
Kalabova, K. | Vorlova, L. | Smutna, M. | Vecerek, V. (Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie) | Borkovcova, I.
In this study the method of HPLC for the determination of hydroxymethylfurfural (HMF) in honey was introduced and validated. The comparison of the HPLC method with classical Winkler's method indicated significant differences in the results determined by both methods (P0.05). It was also found out that the HPLC method provided more accurate results than classical Winkler's method. Employing the established HPLC method we determined the content of HMF in the package of 56 samples of the honeys collected between 1999 and 2002. HMF concentrations varied from 24.8 to 66.1 mg/kg in 1999, from 15.2 to 38.7 mg/kg in 2000, from 5.7 to 38.4 mg/kg in 2001. HMF in the honey from 2002 ranged between the values lower than the limit of detection up to the concentrations of 24.2 mg/kg. The differences between the years were significant (P0.01), except the differences between the years 2001 and 2002. The limit of 40 mg/kg determined by the Czech legislation for the HMF concentration in honey was exceeded in 55.6 % of the samples collected in 1999.
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