Isolation, identification and characterization of rabbit caecal mucinolytic bacteria
2003
Sirotek, K. | Marounek, M. (Akademie Ved, Prague (Czech Republic). Ustav Zivocisne Fyziologie a Genetiky) | Santos, E. | Benda, V.
The aim of our study was to isolate, identify and characterize mucinolytic bacteria from the rabbit caecum. Two hundred and thirty mucin-grown colonies from the caecal contents of 7 rabbits were examined microscopically. Gram-negative irregular rods were the most numerous mucinolytic isolates (34.1 %), followed by gram-negative cocci and short rods (22.5 %). Biochemical characteristics and production of metabolites were used in order to identify 31 isolates. In addition, bacterial cells were hybridized with fluorescently labelled probes for Bacteroides/Prevotella and Clostridium genus. Four isolates were identified at the species level as Mitsuokella multiacidus, three isolates as Bacteroides capillosus and one isolate as Actinomyces izraeli. One strain could be assigned to the Bacteroides/Prevotella genus. Other mucinolytic isolates were not identified as their characteristics did not correspond to any previously described bacterial species. No Clostridium sp. strain was detected. In two M. multiacidus strains high activities of extracellular mucin lyases with pH optimum of 6.2 were found.
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