Effect of additives on coumestrol content in laboratory alfalfa silages
2003
Moravcova, J. | Kleinova, T. | Kvasnicka, F. | Dusek, M. | Cerovsky, M. (Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Chemie Prirodnich Latek) | Loucka, R. | Tyrolova, I.
The effect of inoculant (Lactobacillus plantarum or a mixture of Enterococcus faecium with Lactobacillus rhamnosus), sucrose and formic acid on coumestrol concentration in laboratory alfalfa silages at high moisture content (30 % of dry matter, DM) was investigated. Alfalfa (Morava cultivar) was harvested in May and June 2001, chopped to the length of 30 mm and conserved with additives or without them (control). The number of lactic bacteria, moulds, and yeasts as well as pH and concentration of lactic acid were indicative of adequate preservation. Moulds and yeasts showed no growth except in the first two weeks when slightly aerobic deterioration occurred. The average initial content of coumestrol was established at 213.1+/-29.2 and 207.6+/-7.7 mg/kg DM for the first and second cut, respectively. Its level dropped continually to 20.6+/-9.5 mg/kg DM within 110 days (first cut) and to 44.1+/-10.3 mg/kg DM within 76 days (second cut). The decrease in coumestrol concentration was unaffected by both the treatment and the number of cuts. The acquired data suggest that the residual enzymic activity can be responsible for coumestrol utilization.
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