Production of polyclonal antibodies to bluetongue virus (BTV) and its utilisation for detection of group specific antibodies
2002
Dayakar, S. | Sreenivasulu, D. | Rao, M.V.S. | Sreenivasulu, P. (Sri Venkateswara University, Tirupati (India). Department of Virology)
Bluetongue (BT) is one of the economically important viral diseases established in India. Caused by BTV, a member of the genus Orbivirus, of the family Reoviridae. It affects wide range of domestic and wild ruminants including cattle, sheep, goats, deer and antelopes. However, clinical disease has been mainly confined to sheep. It is more of a problem to exotic sheep than to cross bred sheep. Diagnosis of BT in India is mostly based on symptoms, which is often misleading. Furthermore, cattle and goats act as virus reservoirs. Though, agar gel immuno diffusion (AGID) test is widely used for the detection of BTV antibodies, it also has its own demerits. To overcome these problems, ELISA-based assays using virus protein (VP7) specific monoclonal antibodies were standardized for the estimation of group specific antibodies. Monoclonal antibodies are expensive and needs importation. In this study we describe the production of polyclonal antibodies to purified BTV and showed its utility in estimating the BTV group specific antibodies by cELISA.
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