Application Of RAPD markers for genetic distance analysis of hybrid rice parental lines
2002
Naghia, P.T. | Malik, J.P.S. | Pandey, M.P.Singh, N.K.(Govind Ballabh Pant University of Agriculture and Technology, Pantnagar(India). Department of Genetics and Plant Breeding)
The genetic similarities of 19 hybrid rice parental lines were estimated using RAPD markers. Molecular genetic distance derived from dice's similarity coefficients were based on 133 RAPD markers. Cluster dendrograms were generated for molecular genetic distances. Genetic variability among thermo-sensitive genetic male sterile (TGMS) lines was much lower than that among cytoplasmic genetic male sterile (CMS) lines. PCR analysis based on 10 RAPD primers detected sufficient polymorphisms for the germplasm characterization and genetic distance. The cluster analysis based on these markers revealed close genetic relationship among rice genotypes currently used in the hybrid rice breeding programmes.
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