Seroepidemiological study of bluetongue virus using AB-ELISA
2002
Bhalodiya, M.B. | Jhala, M.K. (Veterinary College, Anand (India). Department of Veterinary Microbiology)
Bluetongue (BT) is an insect transmitted viral disease of several species of domestic and wild ruminants. In many countries like India having considerable sheep population, the disease has becime endemic. Several tests have been developed for the detection of antibodies to BTV serogroup antigen including agar gel immuno deffusion (AGID) test, Indirect ELISA and competitive ELISA Although, no reports were found in literature in employing Avidin-Biotin ELISA (AB-EKUSA) for BT antibodies, its usefulness in detecting antibodies to other viral pathogens have been well documented. BT has been recognized as a disease entity in India for more than 30 years. Subsequent to many wide spreqd epidemics during the after 1981, the disease has now estabkished in India as a predominant sheep disease. Although many serosurveys indicated the presence of BTV antibodies in cattle, buffaloes, goats and camels, clinical disease has not been observed in these species. However, these animals may play an important role in maintence of the virus in nature. The presence of BTV group specific antibodies in cattle becomes a serious impediment to the export of the products from these animals. The absence of vaccination programme against BT in India implies that the occurrence of BTV antibidies in the sera of ruminants has arisen as a consequence of natural exposure to the virus. Although charidel and Hinsu have confirmed existence of BTV in Gujarat, South Gujarat region and some areas of Saurastra regions remained untouched in their studies, so also the cattle population. Hence, these areas were particularly targeted in the present study along with equal/more emphasis on cattle population.
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