Comparative sensitivity of ELISA with DIA and IHA for detection of antibodies against pasteurella multocida
2001
Singh, R. | Jayaprakasan, V. (College of Veterinary and Animal Science, Mannuthy (India). Department of Microbilogy)
Enzyme linked immunosorbent assay (ELISA) which was introduced and developed by Engvall and Perlmann (1972) was considerd as the most sesitive and rapid assay several researches have developed in both chicken and turkey but not so far in ducks to determine antibodies to Pasteurella. Elisa has certain disadvantages such as potential alteration of the antigen conformation upon binding to plastic, non specific adherence of some antibodies to plates and variable cpacities of different antigens present in complex mixture to bind to the microtitre plates. These disadvantages are reported to be circumvented by dotting antigen to diazobenzyloxymethyl membrane or Nitrocellulose membrane, as adorption of the antigens on the membrane, as adsorption of antigens on the membrane is more uniform for longer time than the microtitre plates. Considering the field applicability and advantages over plate ELISA, a comparative study was undertaken to validate and elucidate the significance of dot immuno binding assay (DIA). The present communication compares the indirect haemagglutination (IHA) test with that of plate ELISA and DIA for ducks to determine the humoral antibodies against P. multocida.
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