Production of Herbicide-resistant Transgenic Plants from Embryogenic Suspension Cultures of Cucumber

Woo, J.W.; Jeong, W.J.

Production of Herbicide-resistant Transgenic Plants from Embryogenic Suspension Cultures of Cucumber

2001

Woo, J.W. | Jeong, W.J. (Korea Research Institute of Bioscience and Biotechnology, Daejon, Korea Republic)

To develop herbicide-resistant cucumber plants (Cucumis sativus L. cv Green Angle) embryogenic suspension cultures were co-cultured with Agrobacterium tumefaciens strain LBA4404 carrying a disarmed binary vector pGA-bar. The T-DNA region of this binary vector contains the nopalin synthase/neomycin phosphotransferase ¥+/- (npt ¥+/-) chimeric gene for kanamycin resistance and the cauliflower 35S/phosphinothricin acetyltransferase (bar) chimeric gene for phosphinothricin (PPT) resistance.

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