Production of hGM-CSF from Cell Suspension Culture of Transformed Lettuce Using Agrobacterium-mediated Transformation System
2003
Kim, Y.S. | Kim, M.Y. | Kwon, T.H. | Yang, M.S. (Chonbuk National University, Chonbuk, Republic of Korea)
Lettuce was transformed with Agrobacterium tumefacience LBA4404 containing human granulocyte macrophage colony stimulating factor gene to produce in cell suspension cultues. Cell suspension culture was established using callus from transgenic lettuce plant. Integration of hGM-CSF gene into plant chromosome was confirmed through genomic PCR and Southern blot analysis. In addition, Northern blot analysis indicated the expression of the introduced hGM-CSF gene in transformed lettuce.
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书目信息
Korean Journal of Plant Biotechnology
卷
30
期
1
ISSN
1598-6365
页码
pp. 97-102
其它主题
Celulas
语言
注释
Summary (En)
6 illus; 0 table; 19 ref
类型
Summary
2004-08-15
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