Reliable and economical inoculum production method and disease resistance evaluation techniques to Stenocarpella macrospora in maize
2002
Pascual, C.B. | Guzman, P.S. | Salazar, A.M. (Philippines Univ. Los Banos, College, Laguna (Philippines). Dept. of Agronomy)
The availability of reliable screening method is the cornerstone of any resistance breeding program. Currently, the constraints in the evaluation of maize resistance to disease complex caused by Stenocarpella macrospora are the effective inoculum production protocol and the large-scale screening methods for these diseases. At IPB, the authors developed simple, reliable and inexpensive methods of inoculum production and resistance evaluation technique against S. macrospora. Among the techniques that were tried, the most efficient and reproducible method for large-scale disease screening was the use of corn husk substitute. The inoculum dose, the part of the ear to be inoculated and the optimum time for inoculation were determined. Inoculation was done by simply placing a pinch of corn husk loaded with the organism into the whorl, inserting the substrate in between the stem and ear and in between the third basal stem and leafsheath for leaf blight, ear or and stalk rot resistance evaluation, respectively. The corn inbreds and the hybrids that were screened for resistance to S. macrospora showed disease reaction ranging from resistant to susceptible. This is the first report on the use of corn husk as substitute to produce pure culture of S. macrospora for mass inoculum production and for disease resistance evaluation
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