Response of cowpea germplasm to virus infection
2002
Bashir, M. | Ghafoor, A. | Ahmad, Z. (National Agricultural Research Centre, Islamabad (Pakistan). Plant Genetics Resources Inst.)
Cowpea germplasm consisting of 94 accessions of diverse origin were evaluated for virus infection under field conditions. Virus disease incidence under natural infection conditions ranged from 0 to 66%. Based on ELISA and PCR results following five viruses: cucumber mosaic cucumovirus (CMV), bean common mosaic V), blackeye cowpea mosaic (BICMV) and cowpea aphid-borne mosaic potyviruses (CABMV) and mungbean yellow mosaic geminivirus (MYMV) were detected. Out of 94 lines tested, 18 (19%), 45 (47.8%), 49 (52.1%) and 55 (58.5%) were found infected with CMV,BICMV, BCMV and CABMV respectively. The natural occurrence of BCMV, CMV & YMV on cowpea in Pakistan seems to be the first report. Only 10 accessions (five local:27005, 29154, 27181, 27196 and 27197 and five exotic: IT97K-89235, IT94K556- 6, IT96K-113-6, IT85F-1380, and IT95K-1985) were found free of visible symptoms and no virus was detected in these lines by ELISA. These cowpea genotypes may serve as resistant sources for breeding programme.
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