Induction of xylanase and cellulase genes from Trichoderma harzianum with different carbon sources
2003
Ahmed, S. | Qurrat-ul-Ain | Aslam, N. | Naeem, S. (University of Agriculture, Faisalabad (Pakistan). Molecular Biochemistry Lab.) | Sajjad-ur-Rahman | Jamil, A.
Xylanases and Cellulase are industrially important enzymes obtained from various microorganisms. Trichoderma harzianum is a preferred microorganism for the production of complete xylanases and Cellulase. E-58 strain of the fungus was grown in different media including Enzyme Production Medium (EPM) and Vogel's medium with different carbon sources like xylan, glucose and CMC to get the optimal production of xylanases and cellulases. It was found that glucose repressed the synthesis of these enzymes whereas xylan and CMC produced these enzymes in substantial amounts. Maximal activity of Cellulase and xylanases was marked at pH 5.5 and at temperature 28 degree C. The fungus was grown for 5 days at 120 rpm in orbital shaker. Maximum enzyme activity of xylanase was found to be 1.209 IU mL-1 whereas maximum activity of cellulase (exoglucanase, endoglucanase and bita-glucosidase) was found to be 2.764, 14.4 and 1.1209 IU mL-1 respectively.
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