Analysis of relations between polymorphism in ryanodine receptor gene (RYR1) and certain characters of boar semen in Polish landrace pigs
2003
Kmiec, M. | Dybus, A. (Szczecin Agricultural University (Poland). Dept. of Genetics and Animal Breeding) | Wierzbicki, H. (Wroclaw University of Agriculture (Poland). Dept. of Genetics and Animal Breeding) | Terman, A. | Ziemak, J. (Szczecin Agricultural University (Poland). Dept. of Genetics and Animal Breeding)
The study was carried out in the pedigree herd of 41 boars of the Polish Landrace breed kept at the Breeding and Insemination Station. During the reproduction period 3388 ejaculates were taken and subject to analysis and assessment and the semen concerned was used to inseminate sows serviced by the station. The PCR-RFLP method was used to determine the frequency of mutation 1843C-T in gene RYR1, the said mutation being responsible for stress susceptibility of pigs. In the herd concerned genotype (RYR1**N)(RYR1**N) occurred at the frequency of 0.7317, genotype (RYR1**N)(RYR1**n) at the frequency of 0.2683, whereas genotype (RYR1**n)(RYR1**n) (animals susceptible to stress) was not found. Relations between RYR1 (RYR1**N)(RYR1**N) and (RYR1**N)(RYR1**n) genotypes and qualitative and quantitative characters of semen such as ejaculate volume, concentration of sperm cells, a share of normozoospermia, the number of sperm cells in a dose and the number of insemination doses. The analysis of relations between RYR1 genotypes and the boar semen traits showed the clear and statistically significant (p = 0.01) predominance of the boars of (RYR1**N)(RYR1**N) genotype over the ones exhibiting (RYR1**N)(RYR1**n) genotype in terms of ejaculate volume, share of normozoospermia, the number of normozoospermia in an ejaculate and the number of obtained insemination doses
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