Study on an appropriate culturing time for in vitro maturation of bovine oocytes
1997
Suporn Katawatin | Varocha Jamparat | Monchai Duangjinda | Suchint Simaraks | Thevin Vongpralub (Khon Kaen Univ., Khon Kaen (Thailand). Faculty of Agriculture. Dept. of Animal Science)
Study was conducted to determine an appropriate culturing time for the in vitro maturation of bovine oocytes. The ovarian oocytes of cattle, collected at local slaughter house, were allocated into 3 groups according to the culturing time including 24, 27, and 30 hours. The oocytes were cultured in TCM-199 with 10 percent FCS and incubated at 39 deg C, 5 percent CO2 and high humid. Development of oocytes to metaphase 2 was acriterion for the maturation of oocytes. Results showed that the maturation rate of bovine oocytes cultured for 24, 27, and 30 hours were 4.08, 48.00, and 51.79 percent, respectively. The maturation rate of oocytes cultured for 24 hours was significantly lower (P0.05) than those of 27 and 30 hour-cultures, but there was no difference (P0.05) between those of oocytes cultured for 27 and 30 hours. Based on the time consumed and the quality of oocytes, it was concluded that 27 hours of in vitro culturing was the appropriate time for in vitro maturation of bovine oocytes.
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