Fresh spermatozoa qualities and fertilization rate of European common carp (Cyprinus carpio) by cryopreservation spermatozoa
1997
Meng-umpan, K. (Maejo Univ., Chiang Mai (Thailand). Faculty of Agricultural Production. Dept. of Fisheries Technology)
Spermatozoa cryopreservation plays an important role in aquaculture development as a gamete conservation and fish breeding program. The experiment involving fresh spermatozoa qualities was conducted in terms of density and volume between the pituitary gland of the injected and uninjected male fish. Average spermatozoa densities of 24 and 14 million per cubic mm with 66 and 48 percent of sperm per plasma, were obtained from uninjected and injected male, respectively. The average total sperm volume of 14.2 ml/kg and 2.14 ml/kg of sperm per plasma was obtained from injected male and uninjected male, respectively. Fresh sperm density of 10 million/cubic mm was conducted for spermatozoa cryopreservation. Highest fertilization rates were obtained from Extender 3 (59 and 34 percent) followed by Extender 2 (34 and 30 percent), Extender 4 (34 and 27 percent) and Extender 1 (20 and 14 percent) in straw and pellet, respectively. Meanwhile, fertilization rate from the control was 52 percent. Statistical analysis was highly significant (P0.01) between 3 groups: Group 1 (Extender 3 and control) with Group 2 (Extender 2 and 4) with Group 3 (Extender 1). Frozen sperm motility was the highest in Extender 3 (40 percent) followed by extender 2 (30 percent), Extender 4 (30 percent) and 1 (10 percent). The correlation between sperm motility rate and fertilization rate and showed positive relationship (r
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