Study of suspension culture in Wai Takathong (Calamus caesius Blume.)
1996
Piyarat Parinyapong | Pornchai Chutamas | Kamolpun Namwongprom (Royal Chitralada Projects, Bangkok (Thailand))
Cell suspension culture of Wai Takathong in Murashige and Skoog (MS) liquid medium which supplemented with 2,4-D or picloram at 0, 1, 5, 10, 15 and 20 mg/l. The result showed that 5 mg/l 2,4-D or 20 mg/l picloram the highest number of cell per volume, packed cell volume and cell viability. In MS medium supplemented with 5 mg/l 2,4-D or 20 mg/l picloram and 30, 40 and 50 g/l of sucrose, every concentration of sucrose showed the tendency to increase number of cell per volume and packed cell volume. But 50 g/l sucrose gave the highest cell viability. Cell suspension was cultured in MS liquid medium supplemented with 5 mg/l 2,4-D or 20 mg/l picloram, 50 g/l sucrose and 0, 100, 200, 300 and 400 mg/l L-glutamine. The results showed that 400 mg/l L-glutamine gave the highest number of cell per volume and, packed cell volume and cell viability. When cell suspension of Wai Takathong was cultured in MS liquid medium supplemented with 5 mg/l 2,4-D or 20 mg/l picloram, 50 g/l sucrose and 0, 100, 200, 300 and 400 mg/l casein hydrolysate. It was found that cell viability was increased when MS liquid medium with 5 mg/l 2,4-D, 50 g/l sucrose and 200 mg/l casein hydrolysate were used, but the highest cell viability was found when 20 mg/l picloram, 50 g/l sucrose and 300 and 400 mg/l casein hydrolysate were used in MS liquid medium and casein hydrolysate showed the result better than L-glutamine. The suitable medium for culturing cell suspension was MS liquid medium supplemented with 20 mg/l picloram, 50 g/l sucrose and 300 mg/l casein hydrolysate increase number of cell per volume, packed cell volume and cell viability.
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