Nham product development using mixed bacterial starter cultures 8. Effect of phosphates on the growth of bacterial starter cultures used in Nham production
1995
Pairote Wiriyacharee | Lakkana Rujanakraikarn | Suthaya Boonthanom | Wiwat Wattanatchariya | Issarapong Pongsirikul (Chiang Mai Univ., Chiang Mai (Thailand). Faculty of Agro-Industry. Dept. of Product Development Technology)
Three types of phosphate, used for study on the effect of those compounds on the growth of starter cultures used in Nham production, were sodium tripolyphosphate (0.3 percent) sodium hexametaphosphate (0.3 percent) and sodium acid pyrophosphate (0.3 percent). For this study, selective media MRS wsa used for L. plantarum and P. cerevisiae and selective media BHI for M. varians. The system was also controlled by incubation at 30 deg C for 48 hours. It was found that sodium acid pyrophosphate and sodium hexametaphosphate had an effect on the decrease in pH and increase in total acidity at the initial of incubation. But sodium tripolyphosphate had a similar result with control sample. Additionally, the acid production rate of L. plantarum was higher than P. cerevisiae. However, it was also found that those phosphates (three types) did not significantly affect on the growth of L. plantarum and P. cerevisiae. On the other hand, sodium acid pyrophosphate and sodium hexametaphosphate did decrease significantly on the growth of M. varians at P0.05. While sodium tripolyphosphate did not significantly affect on the growth of M. varians. This culture could grow at normal state and could efficiently reduce nitrite to nitrite in the media system.
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