Establishment of experimental conditions on random amplified polymorphic DNA (RAPD) analysis of Lansium domesticum Corr. II. primer screening and identification of longkong, langsat and duku
2001
Suvimon Konlasuk | Charassri Nualsri | Sompong Te-chato (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
RAPD patterns of Lansium domesticum Corr. were analyzed. Total genomic DNA was extracted from 12 plants each of longkong, langsat and duku from the department of Plant Science, Prince of Songkla University, Songkhla, Pattani and Narathiwat provinces. One of each was sampled for primer screening. Of 100 decamer oligonucleotide primers screened, 47 primers generated polymorphic DNA fragments but only 10 primers (OPA-01, OPB-04, OPB-07, OPC-04, OPC-05, OPC0-08, OPD-01, OPD-03, OPT-01 and OPT-08) showed clear and intense polymorphic bands between longkong, langsat and duku. These 10 primers were then used to identify and detect genetic variation in these 36 plants. No variation in RAPD pattern was observed in the longkong population, indicated its genetic uniformity. There were some differences in RAPD patterns within both the langsat and duku populations indicating their genetic variability. Based on the DNA fingerprint obtained from this study, longkong could clearly be distinguished from langsat and duku.
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