Screening and identification of effective thermotolerant lactic acid bacteria producing antimicrobial activity against Escherichia coli and Salmonella sp. resistant to antibiotics
2000
Sunee Nitisinprasert | Veeraphol Nilphai | Phunjai Bunyun | Prakit Sukyai | Doi, Katsumi | Sonomoto, Kenji (Kasetsart Univ., Bangkok (Thailand). Faculty of Agro-Industry. Dept. of Biotechnology)
Two hundred fifty six isolates of lactic acid bacteria (LAB) displaying antimicrobial activity were isolated from chicken intestine. Twenty effective isolates of LAB show bacteriocin-like activity (BLA) against the target strains resistant to antibiotics (Escherichia coli and Salmonella sp.) to varying degrees. Broad spectrum inhibition specific (SIS) were found from 6 h cultivation while the longer cultivation time of 12 and 24 h appeared either the loss of BLA or the inhibition activity against the different target strain. Only 3 effective thermotolerant isolates, i.e., KUB-AC5, KUB-AC16 and KUB-AC20, exerted broad SIS against all target strains except Salmonella sp. S001. However, BLA belonging to KUB-AC5 could not inhibit growth of E. coli E006 and E010 whereas KUB-AC20 did not inhibit only E. coli E006. Based on biochemical, physiological and molecular systematic method, these isolates were classified as Lactobacillus reuteri.
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