Inoculum preparation affecting on monascus yellow pigment production
1993
Somchai Krairak | Busaba Yongsmith | Pramote Sirirote (King Mongkut's Institute of Technology Ladkrabang, Bangkok (Thailand). Faculty of Science. Dept. of Applied Biology)
Improvement of Monascus kaoliang KB 9 resulted a secondary mutant strain KB 20M10.2: which is capable on producing higher yellow pigmentation at single wavelength (lambda 370 nm) but slower growth and sporulation. Usually inoculum preparation using rice koji was thus found unproper for this mutant. Current trend in inoculum preparation using liquid type was developed. Various factors such as inoculum ages (1, 2, 3 and 4 cultivated-day-old), inoculum size (1.0 percent, 2.0 percent and 3.0 percent v/v), homogenized inoculum cells at various time (0-2 min) affection on yellow pigment fermentation were studied. The results showed that 2 minutes homogenized cells of 3-day old at 1.0 percent v/v gave the maximal pigments about 530 U/ml with thread-like pellet from mycelial growth and rather viscous medium. Centrifuged or uncentrifuged cells inoculum gave similar pigmentation at 496 and 487 U/ml with disc and needle pellet from mycelial growth, respectively. Whereas koji inoculum (3.0 percent) w/v) of 30-day old gave only 431 U/ml yellow pigmentation. Liquid inoculum (non-homogenized and uncentrifuged cells) could thus reduce the inoculum preparation time and amount from 4 weeks and 3.0 percent (w/v) of koji inoculum to 2-day old and 3.0 percent (v/v) of uncentrifuged cells and gave higher yellow pigmentation.
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