Fatty acids analysis by packed column and capillary column
2000
Kunchit Judprasong (Mahidol Univ., Bangkok (Thailand). Institute of Nutrition)
Saturated fatty acids is one of the mandatory nutrients for nutrition labelling, according to the Thai Notification of Ministry of Public Health, number 182 (B.E.2541) and the US-FDA nutrition labelling regulation. A gas-liquid chromatographic (GLC) method using a packed column has been commonly used for quantitative measurement of fatty acids in foods. Fatty acid content in 60 food items, determined quantitatively by acid hydrolysis-GLC with packed column, were compared with the contents determined by GLC with capillary column. For all food products studied, the amount of saturated fatty acid and the profile of all fatty acids separated by the two types of column were not significantly differnt (p0.05). The saturated fatty acid analysed by both column showed linear relationships with a slope of 0.986, while the fatty acid profile had the correlation coefficient of greater than 0.8 except those of linolenic, arachidic and eicosenic (r = 0.64, 0.18 and 0.62 respectively). Participation in the ASEAN FOODS laboratory performance studies showed satisfactory results (z score2) for saturated fat analysis by GLC using capillary column. For internal quality assessment, the results of repeated extractions and analysis of fish powder suggested that the material could be used as an in-house quality control sample for saturated fatty acid.
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