Use of 15N-dilution technique and acetylene reduction assay to test the effectiveness of some Bradyrhizobium japonicum strains on soils with different pH
1998
Chantana Siripaibool | Pornpimol Chaiwanakupt | Chitima Yathaputanon | Jittra Claimon | Wimon Pinphaithun | Mvula, A.B. (Department of Agriculture, Bangkok (Thailand). Agricultural Chemistry Div.)
Pot experiment was conducted to determine relationship between two measurements of N2 fixation using soybean and to uses these measurements to evaluate a number of Bradyrhizobium japonicum strains for effectiveness in N2 fixation at soil pH 5.2 and 7.0. The results showed that preliminary screening of strains was as effective method of selecting strains for the use in field test. 15N dilution measurement of N2 fixation showed that levels of fixation are ranging from 4.43-7.79 mg N/pot depending on bacterial strains and soil pH. Acetylene reduction assay (ARA) proved to be of little value for screening B. japonicum strains. These results emphasize the need for caution in using ARA in the final screening of B. japonicum strains for N2 fixing potential.
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