Tissue culture propagation of gloxinia
1996
Mayuree Wuttisit | Kamnoon Kanchanapoom (Hat-Yai Vidhayalai School, Songkhla (Thailand))
Rapid propagation of Gloxinia (Sinningia speciosa Benth. & Hook.) was achieved by means of leaves, stem segments and shoot tips culture in vitro. These explants were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of BA, NAA or 2,4-D. Efficient shoot regeneration obtained from leaf was better than other explants. Ms medium containing Ba, NAA and combination of BA and NAA at 1 mg/l each produced 69, 47.5 and 92 shoots per leaf explant, respectively. The addition of 2,4-D in the MS medium did not promote the formation of shoots but enhanced callus growth and differentiation. After several subcultures of these calli on the same medium, multiple shoot production was evident. For root initiation, exogenous auxins were found to be necessary for in vitro-grown shoots. Regenerated shoots were transferred to MS medium supplemented with either IAA, IBA, NAA or hormone-free medium. The highest number of 40 roots per shoot explant was obtained from cultures grown on medium containing 3 mg/l IBA. Complete plantlets were acclimatized and successfully transplanted to glasshouse conditions.
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