Studies on possible detection of mammalian metallothionein in higher plant by radioimmunoassay
1994
Praphasri Chongpraditnun | Otaki, Noriko | Chino, Mitsuo (Department of Agriculture, Bangkok (Thailand). Soil Science Div. Soil Chemistry Research Group)
Metallothionein (MT) is low molecular weight, cysteine rich, heavy metal hinding protein found in animals, Neurospore and fusion yeast but not in fission yeast. Recently, MT genes from plants, for instance; flowering plant; Nimutus guttatus, pea; Pisum sativum L., maize and soybean have been cloned. Although these plant genes with homology to mammalian MT genes were found, the information on plant MT protein is still unknown. Using Radioimmunoassay (RIA) technique showed a possibility of MT synthesis in plants. Radioimmunoassay for MT in soybean suggested that MT like protein was contained at the concentrations of 427.7 and 7,700 ng/g fresh weight by rat-MT equivalence, in roots and seeds, respectively (conc. 0.025 percent of total protein). While the rice roots and wheat grains did not contain this MT like protein.
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