Fish propagation in brown trout (Salmo trutta) by spermatozoa cryopreservation using four extenders
1994
Kriangsak Mengumpan | Stein, Heibert | Mathes, Elisabeth (Maejo Institute of Agricultural Technology, Chiang Mai (Thailand). Faculty of Agricultural Production. Dept. of Fishery Technology)
This study on the effect of spermatozoa cryopreservation on the rate of fertilization in Brown Trouts (Salmo trutta) was conducted at the Fish Biology Section of the Department of Zoology, Technical University of Munich, Germany, during November 15, 1993 to February 15, 1994. The first factorial experiment compared the use of four extenders for cryopreservation in pellets and straws. All extenders contained DMSO, in addition extender 1 contained NaCl and egg yolk extender 2 contained NaCl and no egg yolk, extender 3 contained NaCl and high glucose and extender 4 contained high glucose and egg yolk. The rate of fertilization was highest in extender 4 at 52 percent (pellet) followed in extender 3 at 48 percent (pellet) and in extender 1 (straw) at 33 percent Statistical analysis showed a significant difference between extenders (P0.01). Analysis of the averages with Duncan's Multiple Range Test (DMRT) showed that pellets gave better fertilization rates than straws. The second experiment studied the use of 0.8 percent NaCl during repeated fertilization with sperm frozen in extender 1. Fertilization rates ranged between 75 and 81 percent and showed no significant differences. The third experiment compared the use of the four extenders in straws. Results showed fertilization rates at 55, 43.9, 43.4 and 22 percent in extenders 1, 2, 4 and 3 respectively. No significant difference was observed among extenders 1, 2 and 4 as shown by DMRT test, however, extender 3 less effective.
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