Phenotypic characteristics of thymic microenvironment in WR-638-protected rats after whole-body irradiation: macrophages, interdigitating cells and mesenchymal stroma
Dragojevic-Simic, V.(Military Medical Academy, Belgrade (Serbia and Montenegro). National Poison Control Centre)E-mail:[email protected] | Gasic, S.(Military Medical Academy, Belgrade (Serbia and Montenegro). Institute of Medical Research) | Colic, M.(Military Medical Academy, Belgrade (Serbia and Montenegro). Institute of Medical Research) | Bokonjic, D.(Military Medical Academy, Belgrade (Serbia and Montenegro). National Poison Control Centre)
Phenotypic changes of macrophages, interdigitating (ID) cells and mesenchymal stroma of the thymus were studied in male Wispar rats subjected to radioprotector WR-638 (358 mg+AFw-kg b.w. ip) and/or whole body X-ray irradiation 15 days after their application. They were identified in situ on cryostat thymic sections using immunoperoxidase staining by a R-MC 23, R-MC 39, R-MC 41, R-MC 44, R-MC 45, ED1, ED2 and OX 39 monoclonal antibodies (mAbs). It was shown that WR-638 protected rat thymus from ionising radiation effects, preferentially as a result of lymphoid compartment preservation. However, radioprotector also caused changes in the number and phenotypic characteristics of macrophages and ID cells in irradiated rat thymus. WR-638 prominently reduced changes caused by radiation in vascular structures of the thymus, too. This influence of WR-638 on thymic microenvironment elements probably contribute to its beneficial radioprotectove effect on the irradiated rat thymus
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