Immobilized lipases as practical catalysts. [Doctoral dissertation]
2004
Knezevic, Z.
The various immobilization procedures, reactor configurations and process considerations are all reviewed and discussed. The properties of the immobilized lipases are considered and related to the lipase performance both in terms of activity and stability. Applications of immobilized lipases in the chemical, pharmaceutical and food industries as well as probable future trends in lipase catalyzed processes are also discussed. Special attention is given to systems based on lipase adsorbed on macroporous polymer beads or entrapped in hydrogel such as alginate microbeads. It appears that lipase from Candida rugosa immobilized on a Cuprophane membrane in a hollow fiber modele was the most productive and stable system in palm oil hydrolysis. The proposed immobilization technique and hollow fiber reactor system with immobilized lipase were shown to be suitable for oil hydrolysis and attractive for further exploration in long-term applications. Features associated with immobilized lipase kinetics such as enzyme activities, adsorption properties, optimum operating conditions, and estimates of the specific parameters in kinetic formulations are also presented in the book.
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