Thin-layer chromatography analysis and scavenging activity of marigold (Calendula officinalis L.) extracts
2003
Cetkovic, G.S. | Djilas, S.M. | Canadanovic-Brunet, J.M. | Tumbas, V.T.(University of Novi Sad, Novi Sad (Serbia and Montenegro). Faculty of Technology)
The methanol, petroleum ether, chloroform, ethyl acetate, n-butanol and water extracts were obtained by extraction of marigold flower (Calendula officinalis L.). The content of total phenolic compounds, determined by UV spectrophotometric method using the Folin-Ciocalteu reagent, was 15.12 mg/g. The content of total flavonoids, determined by UV spectrophotometric method according to Markham, was 5.13 mg/g. Qualitative determination of phenolic compounds in the extracts was performed by one- and two-dimensional thin-layer chromatography (TLC) procedures. The results of one- and two-dimensional TLC analyses showed that different flavonoids and phenolic acids were present in the investigated extracts. The greatest number of flavonoids (rutin, quercetin and some unidentified flavonoid glycosides) and phenolic acids (chlorogenic, caffeic, coumaric and vanillic acid) were determined in methanol extract.
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