RAPD analysis in Crocus sativus L. accessions and related Crocus species
Grilli Caiola, M.(Universita di Roma (Italy). Dipartimento di Biologia)E-mail:[email protected] | Caputo, P.(Universita di Napoli Federico II (Italy). Dipartimento di Biologia Vegetale) | Zanier, R.(Universita di Roma (Italy). Dipartimento di Biologia)
RAPD investigation was carried out on DNAs from five Crocus sativus accessions cultivated in different countries and on six closely related Crocus species. Aims of the study were to check whether cultivated saffron has maintained a constant genomic organisation and to clarify its relationships with possible ancestor species. For the fifteen primers which produced positive results, DNAs of saffron corms from different accessions present the same amplification pattern, in accordance with the similar DNA concentration and base composition pointed out in previous studies. The amplification of the seven Crocus species DNAs with twenty-one primers provided 217 repeatable and intrpretable fragments which were scored for presence/absence and employed for a cluster analysis. Results indicated that C. sativus is very closely related to C. cartwrightianus and also similar to C. thomasii. This result would rule out the hypothesis of close relationships between C. sativus and C. pallasii.
显示更多 [+] 显示较少 [-]