The evaluation of cadmium, zinc and nickel accumulation ability of transgenic tobacco bearing different transgenes
Pavlikova, D.(Ceska Zemedelska Univ., Prague (Czech Republic). Agronomicka Fakulta)E-mail:[email protected] | Macek, T.(Akademie Ved, Prague (Czech Republic). Ustav Organicke Chemie a Biochemie) | Mackova, M.(Akademie Ved, Prague (Czech Republic). Ustav Organicke Chemie a Biochemie) | Sura, M.(Akademie Ved, Prague (Czech Republic). Ustav Organicke Chemie a Biochemie) | Szakova, J.(Ceska Zemedelska Univ., Prague (Czech Republic). Agronomicka Fakulta) | Tlustos, P.(Ceska Zemedelska Univ., Prague (Czech Republic). Agronomicka Fakulta)
Nicotiana tabacum var. Wisconsin 38 as the control (WSC) and four genetically modified lines of the same variety were tested for Cd, Zn and Ni accumulation. Genetically modified lines of the same variety, bearing the transgene CUP1 (gene coding a yeast metallothionein), GUS (reporter gene for beta-glucuronidase), HisCUP (CUP combined with a polyhistidine tail), and HisGUS (reporter gene for beta-glucuronidase, combined with a polyhistidine tail) under a constitutive promoter, enabling it to follow the heavy metal tolerance and uptake changes as a function of the transgene present. Control and transgenic lines were tested for accumulation of risk elements on sand nutrient medium with the addition of Ca, Zn and Ni. The results showed high Cd accumulation ability of HisCUP line. The Cd concentration in aboveground biomass was increased by 90% in comparison with the non-transformed control, whereas Cd concentration in roots was decreased by 49%. The changes in Zn concentration in aboveground biomass were not significant, but Ni concentration increased significantly in aboveground biomass of HisGUS construct. GUS construct introduced the ability to accumulate all investigated metals.
显示更多 [+] 显示较少 [-]