Organic pollutant contamination of the river Elbe as assessed by biochemical markers
Siroka, Z.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie)E-mail:[email protected] | Krijt, J.(Karlova Univ., Praha (Czech Republic). Ustav Patologicke Fyziologie) | Randak, T.(Jihoceska Univ., Vodnany (Czech Republic). Vyzkumny Ustav Rybarsky a Hydrobiologicky) | Svobodova, Z.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie) | Peskova, G.(Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Fakulta Veterinarni Hygieny a Ekologie) | Fuksa, J.(Vyzkumny Ustav Vodohospodarsky TGM, Prague (Czech Republic)) | Hajslova, J.(Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Chemie a Analyzy Potravin) | Jarkovsky, J.(Masarykova Univ., Brno (Czech Republic). Centrum Biostatistiky a Analyz) | Janska, M.(Vysoka Skola Chemicko-technologicka, Prague (Czech Republic). Ustav Chemie a Analyzy Potravin)
Cytochrome P450 (CYP 450) and ethoxyresorufin-O-deethylase (EROD) were used in order to evaluate the pollution of the river Elbe. The results were correlated with polychlorinated biphenyls (PCB) concentrations in fish muscles, polyaromatic hydrocarbons (PAH) values in bottom sediments and 1-hydroxypyrene (1-OHPY) values in fish bile. Leuciscus cephalus captured at ten locations was selected as the indicator species. A comparison between the EROD activity and the CYP 450 content along the longitudinal profile of the Elbe showed a significant correlation. The highest EROD activity levels in the liver were ascertained in Zelcin (341 pmol/min/mg), whereas in Blanice (control location) EROD activity was significantly lower than in any of the other locations studied. The study failed to produce an unambiguous proof of any correlations between detoxification enzyme activity (CYP 450 and EROD) in the liver and their two important inducers (PCB and PAHs).
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