Production of ligninolytic enzymes of white-rot fungi from Indonesian tropical rainforest and their bleachability on the kraft pulp of Acacia mangium
2002
Prasetya, B. | Idiyanti, T. (Pusat Pengkajian Ilmu Pengetahuan dan Teknologi, Serpong (Indonesia))) | Goenadi, D.H. | Siagian, R.M. | Yoshida, S. | Watanabe, T. | Kuwahara, M.
This work deals of the investigation on the production of ligninolytic enzyme of white rot fungi isolated from natural forest, West Java, Indonesia and their ability for bleaching kraft pulp from Acacia mangium. For producing enzyme, newly isolated fungi were cultivated in wood meal medium and liquid medium using polypepton and glucose. The lignin peroxidase, manganase peroxidase and laccase were determined and their bleachability on the kraft pulp through decolorization of pulp-agar and concentrated chromophore substances extractable from kraft pulp. Furthermore, the bleach-activity directly on the kraft pulp was also investigated. The results showed that the isolated fungi CPN01, PSM01 and CBD06 produced ligninolytic enzyme generally with relatively higher activity compared to common investigated fungi. In wood meal culture medium, the maximum MnP-activity of about 200 unit/5 g dry pulp was found by fungus PSM01, while in liquid culture medium the maximum MnP-activity of approximately 50 Unit/20 ml culture medium was reached by fungus CPN01. From the isolated fungi, remarkable LiP-activity was produced only by fungus CNP01 in wood meal medium and liquid culture medium. CPN01 and PSM01 have ability to bleach the pulp during cultivation on the pulp agar. In addition, CPN01 was also able to decolorize solution containing concentrated chromophore substances. It is noted that fungi CPN01 showed a good possibility for biobleaching. Inoculation directly on the kraft pulp with CPN01 indicated that remarkable decrease of kappa number depending on the nutrient that added to pulp substrate and inoculation method. The maximum brightness of pulp sheet, around 70 percent with kappa number of 8 is found at incubation 15 days inoculated by subculture suspension with addition of 6 percent glucose
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