Use of spermatozoa from epididymus of slaughtered rams for in vitro fertilization of ovine oocytes
2003
Wani, N.A. | Wani, G.M. (S K University of Agricultural Sciences and Technology, Srinagar (India))
The oocytes harvested from the ovaries obtained from a local slaughterhouse were matured in vitro. The oocytes reaching to metaphase II stage were 63.21%. The spermatozoa used in this study were obtained from the cauda epididymus of 2-3 testes collected from local abattoir. The washed sample were having 70-8-% motility, rising up to about 90% after swimup treatment. Sperm motility was maintained throughout the capacitation period in all the 3 media. At the time of insemination above 80% of the spermatozoa exhibited progressive motility in all the 3 media. The oocyte penetration rates were 46.5, 53.4 and 54.5, respectively, for oocytes in TCM-199, Hams F10 and Hams, F10 and Hams F12 respectively. Out of the oocytes incubated further only 7,4 and 5.7% cleaved. It can be concluded that the spermatozoa collected from the eqididymus of dead/slaughtered rams can be used successfully for the in vitro fertilization of occytes in sheep.
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