Plant regeneration via somatic embryogenesis in chickpea (Cicer arietnum L.)
2002
Cauhan, R | Singh, N.P. (Indian Institute of Pulses Research, Kanpur (India))
Efficient plant regeneration via somatic embryogenesis has been developed in chickpea from embryonic axes on MS medium supplemented with 1.25 mg/l 2, 4-D and 0.25 mg/l, kinetin induced callusing after 7 days old callus masses started differentiating with high efficiency of globular structures during dark incubation. « MS salts +B5 vitamins+40 g/l, sucrose was found suitable for embryo maturation. The well formed embryos germinated into platelets on MS salts +B5 vitamins +1.25 mg/l IBA + 2.0 mg/l BAP. Normal plants were transferred to soil.
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