Effect of various growth regulations on the production of protocorm like bodies in three orchid genera
2002
Saiprasad, G.V.S. | Raghuveer, P. | Khetrapal, S. | Chandra, R. (Indian Agricultural Research Institute, New Delhi (India). Division of Plant Physiology)
Efficiency of protocorm like bodies (plbs) production as a function of various growth regulators was asessed in three orchid genera viz. Dendrobium 'soni', Oncidium' Gower Ramsay' and Cattleya leopoldii. Growth regulators viz. BAP, kinetin, NAA, IAA, 2,4-D and GA3 were supplemented to MS medium at concentrations of 0.25, 0.5 and 1.0 mg l-1. The number of days taken for initial differentiation was observed to be a function of growth regulator supplemented as well as orchid genera. Maximum number of plbs were produced in BAP 1.0 mg l-1. The relative ranking among plant growth regulators (highest to lowest ) for obtaining maximum number of plbs was BAP KinetinNAA2,4-DGA3. Similar ranking for theree orchid genera was Dendrobium OncidiumCattleya.
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