In vitro multiplication of phoenix dactylifera (L)
2003
Bhargava, S.C. | Saxena, S.N. | Sharma, R. (Rajasthan Agricultural University, Bikaner (India). Department of Plant Biotechnology)
A method of microcloning via somatic embryogenesis was established in datepalm (Phoenix dactylifera L) cultivars. The method has potential for commercial application. Embryogenic cultures were initiated from soft primordial tissues of 2-3-year-old female suckers. The system was optimized first for the genotype 'Sayar' and then its applicability was tested to other genotypes. A method of pre-acclimation using pre-acclimatin chambers has been devised, which improved hardening survival greatly. More than 80 clones obtained from the genotype 'Sayar' have been shifted to field for agronomic evaluation.
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