Genetic and breeding science of abalone and their application to the aquaculture techniques
2001
Hara, M. (National Research Inst. of Aquaculture, Nansei, Mie (Japan)) | Sekino, M.
To promote genetic researches and breeding science of Pacific abalone, we are beginning to develop the isolation and characterization the hyper-variable markers such as microsatellite DNA. Microsatellite DNA markers are expected to provide an invaluable tool for this purpose because of the power and ability of microsatellite DNA markers in regard to resolution for genetic relatedness among individuals or populations. Here, we describe the applications to the aquaculture techniques of abalone using microsatellite DNA markers. Comparing the genetic variability of microsatellite DNA with that of allozyme in natural and artificial populations, the viability observed in microsatellite DNA analysis was evidently higher than that observed in allozyme analysis. The fact was suggesting that the microsatellite DNA should prove useful as sensitive genetic markers to estimate genetic variability. As the application to the discrimination of individual, the estimation of the traits of pedigree in mixed rearing populations was initiated to establish using five microsatellite DNA markers in Pacific abalone. The group that was strong to withering syndrome was found from survival groups against the disease. The feasibility of pedigree analysis using microsatellite DNA markers could be ascertained. It is considered that the development of abundant number of microsatellite DNA markers in abalone promote the genetics and breeding science and become one of the most useful approaches to develop the aquaculture techniques.
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